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Santa Cruz Biotechnology
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Standard format: Plasmid sent in bacteria as agar stab
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Standard format: Plasmid sent in bacteria as agar stab
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Image Search Results
Journal: Molecules and Cells
Article Title: Dipeptidyl peptidase 4 as an injury-responsive protein in the mouse sciatic nerve
doi: 10.1016/j.mocell.2024.100159
Figure Lengend Snippet: Systemic Dpp4 depletion results in significant behavioral abnormalities in mice and DPP4 is predominantly expressed in nonaxonal cells of the mouse sciatic nerve. (a) Tail suspension assay demonstrating that Dpp4 knockdown mice show hindlimb clasping, indicating impaired neurological function. (b) Open field test results with tracking of mouse movement (red lines). (c-e) Immunofluorescence staining of cross-sections from adult mouse sciatic nerve, showing DPP4 localization in comparison to markers for myelinating Schwann cells (MBP, myelin basic protein, c), nonmyelinating Schwann cells (NCAM1, neural cell adhesion molecule, d), and axons (TUJ1, β3-tubulin, e). DPP4 shows ring-like pattern (arrows) and patch-like pattern (arrowheads). Scale bar, 10 µm (left) or 5 μm (right, magnified). (f) Quantification of ratio of non-TUJ1-overlapping “ring”-like DPP4 staining and TUJ1-overlapping “patch”-like DPP4 staining ( n = 4 of unit areas from the individual mouse sections, **** P < .0001). (g) Embryonic DRG tissues from E12.5 mice were dissected, dissociated, and cultured. The +FDU group represents a pure neuronal culture where fluorodeoxyuridine was added to eliminate dividing non-neuronal cells, while the −FDU group allowed non-neuronal cells to survive alongside neurons (scale bar, 100 µm). (h) Western blot analysis of total lysates collected on day 6 from the cell cultures shown in (g). Cases 1 and 2 represent independent biological replicates.
Article Snippet: The following antibodies were used in the present study: anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody (Santa Cruz Biotechnology, sc-32233, clone 6C5, 0.2 mg/ml, 1:200), anti-βIII tubulin (TUJ1) antibody (Abcam, ab41489, 0.3 mg/ml, 1:300), anti-superior cervical ganglion 10 (SCG10) antibody (Novus Biologicals, NBP1-49461, 1.0 mg/ml, 1:1,000),
Techniques: Suspension, Knockdown, Immunofluorescence, Staining, Comparison, Cell Culture, Western Blot